Device

Part:BBa_K1260011:Design

Designed by: Zhengfeng Liu   Group: iGEM14_Shenzhen_SFLS   (2014-06-08)


pbad promoter and membrane protein LGT (whole device)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1243
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 864
    Illegal SapI.rc site found at 361


Design Notes

The promoter may have a little of leak expression.


Source

It comes from BBa_B0015,BBa_B0030, BBa_K260001 and BBa_K1260002.

References